gfp lentivirus  (Santa Cruz Biotechnology)

Journal: Frontiers in Cellular Neuroscience

Article Title: LAMTOR1 regulates dendritic lysosomal positioning in hippocampal neurons through TRPML1 inhibition

doi: 10.3389/fncel.2024.1495546

Figure Lengend Snippet: Antibodies, chemicals, and plasmids used in this study.

Article Snippet: GFP Control Lentiviral Particles , Santa Cruz Biotechnology , Cat#sc-108084.

Techniques: Virus, Control, shRNA, Plasmid Preparation, Recombinant

Journal: Journal of Virology

Article Title: Interferon-γ inducible factor 16 (IFI16) restricts adeno-associated virus type 2 (AAV2) transduction in an immune-modulatory independent way

doi: 10.1128/jvi.00110-24

Figure Lengend Snippet: Exogenous complementation of IFI16 in U2OS IFI16 -/- cells. U2OS IFI16 -/- were either untransduced, transduced with lentiviral vectors expressing GFP (MOI 5), or transduced with lentiviral vectors expressing IFI16 fused to monomeric GFP (MOI 5) in the presence of polybrene. After 72 hours, the cells were infected with rAAV2mCherry (MOI 500). ( A ) mCherry expression was assessed by RT-qPCR using specific primers for mCherry. ( B ) Exogenous complementation of IFI16 was confirmed on transcript level. Graphs show mean and SD of the relative gene expression from triplicate experiments. P -values were calculated using an unpaired Student’s t -test (* P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001).

Article Snippet: Lentiviral vectors expressing GFP (TR30021V) or IFI16 fused to monomeric GFP (RC202193L2V) were obtained from OriGene (Rockville, USA).

Techniques: Transduction, Expressing, Infection, Quantitative RT-PCR

Journal: Journal of Virology

Article Title: Interferon-γ inducible factor 16 (IFI16) restricts adeno-associated virus type 2 (AAV2) transduction in an immune-modulatory independent way

doi: 10.1128/jvi.00110-24

Figure Lengend Snippet: IFI16 inhibits AAV2 gene expression in an Sp1-dependent manner. U2OS IFI16 -/- and U2OS wt cells were either untransduced (U2OS IFI16 -/- or U2OS wt, respectively), transduced with lentiviral vectors expressing GFP (MOI 5; U2OS IFI16 -/- + GFP ctrl.), or transduced with lentiviral vectors expressing IFI16 fused to monomeric GFP (MOI 5; U2OS IFI16 -/- + IFI16_GFP). After 72 hours, the cells were infected with AAV2 (MOI 20,000) and 24h later subjected to ChIP assays using an anti-Sp1 antibody and primers for the ( A ) p5 or ( B ) p19 promoter regions, respectively. ( C ) Interaction of IFI16 and Sp1 was assessed by co-immunoprecipitation in U2OS wt and U2OS IFI16 -/- cells. Graphs show mean and SD of the relative promoter occupancy from triplicate experiments. P -values were calculated using an unpaired Student’s t -test (* P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001).

Article Snippet: Lentiviral vectors expressing GFP (TR30021V) or IFI16 fused to monomeric GFP (RC202193L2V) were obtained from OriGene (Rockville, USA).

Techniques: Expressing, Transduction, Infection, Immunoprecipitation